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Thermo Scientific™ Oxoid™ Charcoal Agar Base (Dehydrated)
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Specifications:
| Application | Microbiology | ||
| Storage Temperature | Room Temperature | ||
| Product Type | Culture Medium | Forms | Powder |
| Product Brand | Thermo Fisher Scientific™, Oxoid | Target Organism Class | Haemophilus influenzae, Bordetella pertussis |
| Product Grade | Microbiology grade | Formula | For 9.8L medium |
The Thermo Scientific™ Oxoid™ Charcoal Agar Base (Dehydrated) is a specialized medium designed for the cultivation and isolation of Bordetella pertussis and Haemophilus influenzae. This medium provides a non-blood-containing formulation enriched with nicotinic acid, an essential growth factor for Bordetella spp. Its selective properties make it suitable for isolating target organisms from nasopharyngeal secretions while suppressing unwanted flora during long incubation periods.
Key Features
- Blood-Free Medium:
- Facilitates the growth of Bordetella pertussis and Haemophilus influenzae without the need for blood enrichment.
- Nicotinic Acid Addition:
- Acts as an essential growth factor for Bordetella spp., enhancing recovery rates.
- Selective Agents:
- Compatible with selective agents like cephalexin, which suppress unwanted nasopharyngeal flora while promoting Bordetella recovery.
- Wide Application:
- Effective for clinical microbiology workflows targeting respiratory tract infections caused by Haemophilus and Bordetella.
Applications
1. Clinical Microbiology
- Isolation and cultivation of Bordetella pertussis from nasopharyngeal secretions for the diagnosis of whooping cough.
- Cultivation of Haemophilus influenzae from respiratory samples for meningitis and other infections.
2. Vaccine Production
- Used in the growth and cultivation of Bordetella pertussis for vaccine manufacturing.
3. Research Microbiology
- Study of Gram-negative coccobacilli and respiratory pathogens in laboratory settings.
Specifications
| Attribute | Details |
|---|---|
| Product Type | Agar |
| Form | Powder |
| Description | Charcoal Agar Base |
| Selective Agents | Cephalexin, methicillin, or penicillin |
| Enrichment | Nicotinic acid |
| Quantity | 500g |
| Applications | Clinical, research, and vaccine production |
Preparation Instructions
- Suspend: Suspend 30.5g of Charcoal Agar Base in 1 liter of distilled water.
- Mix and Heat: Heat with frequent agitation until fully dissolved.
- Sterilize: Autoclave at 121°C for 15 minutes.
- Supplement: After cooling to 45–50°C, aseptically add selective agents or supplements as required.
- Dispense: Pour the prepared medium into sterile Petri dishes and allow it to solidify.
Advantages
- Enhanced Selectivity: Suppresses unwanted nasopharyngeal flora with selective agents such as cephalexin or methicillin.
- Optimized for Bordetella Recovery: Enriched with nicotinic acid to support robust growth of Bordetella spp.
- Broad Utility: Suitable for clinical diagnosis, research studies, and vaccine production workflows.
- Non-Blood-Based Formulation: Provides reliable performance without requiring blood supplements.
Diagnostic Features
| Organism | Colony Morphology |
|---|---|
| Bordetella pertussis | Small, pearl-like, dome-shaped colonies with a greyish hue. |
| Haemophilus influenzae | Small, transparent, droplet-like colonies. |
Selective Agent Compatibility
| Selective Agent | Function |
|---|---|
| Cephalexin (40 µg/mL) | Suppresses unwanted flora, allowing superior Bordetella recovery. |
| Methicillin | Inhibits non-target nasopharyngeal flora. |
| Penicillin | Original selective agent for Bordetella spp. isolation. |
Storage and Stability
- Storage: Store the dehydrated medium at 10–30°C in a dry environment.
- Shelf Life: Refer to product label for expiration date.
Packaging
- Product: Charcoal Agar Base (Dehydrated)
- Quantity: 500g
The Thermo Scientific™ Oxoid™ Charcoal Agar Base (Dehydrated) is a reliable medium for isolating Bordetella pertussis and Haemophilus influenzae. With its blood-free formulation and enrichment with nicotinic acid, it ensures effective recovery and differentiation of target organisms while suppressing non-target flora. This medium is indispensable for clinical diagnostics, research microbiology, and vaccine production.