Thermo Scientific™ Arcturus™ PicoPure™ RNA Isolation Kit

Arcturus™ PicoPure™ RNA Isolation Kit Overview

The Arcturus™ PicoPure™ RNA Isolation Kit is designed to deliver high-quality total RNA from minute samples, such as fewer than ten cells or even a single cell. This kit ensures superior RNA recovery with minimal input and supports downstream applications like gene expression analysis, microarray analysis, and real-time PCR (qPCR). With a streamlined workflow and specialized MiraCol™ Purification Columns, this kit maximizes RNA yield while maintaining RNA integrity.

Key Features

1. Exceptional Sensitivity:
   • Recovers RNA from as little as a single cell, making it ideal for precious, low-input samples.
2. High Efficiency:
   • Maximizes RNA recovery for gene expression studies, even from RNA-poor samples.
3. Convenient Small Volume Elution:
   • Elutes RNA in as little as 10 μL of buffer, ready for downstream applications without vacuum concentration.
4. Unique MiraCol™ Purification Columns:
   • High-recovery purification columns ensure consistent RNA recovery while protecting against RNase contamination.
5. Flexibility:
   • Compatible with a variety of sample types, including Laser Capture Microdissection (LCM) samples and frozen tissues.
6. Integrated Contamination Prevention:
   • Columns feature built-in lids to maintain an RNase-free environment.
7. Ready for Amplification and Microarray Analysis:
   • Delivers RNA suitable for direct amplification with RiboAmp™ RNA Amplification Kit and other downstream workflows.

Specifications

Applications

1. Gene Expression Studies:
   • Ideal for low-abundance gene analysis using qPCR or microarrays.
2. Small Sample RNA Extraction:
   • Designed for RNA recovery from LCM samples, frozen tissue sections, and other small sample types.
3. RNA Amplification:
   • Prepares RNA for amplification workflows, including the RiboAmp™ RNA Amplification Kit.
4. High-Sensitivity Analysis:
   • Suitable for detecting low-copy-number transcripts.

Advantages

Sample Compatibility

Kit Components

• Extraction Buffer: For efficient RNA extraction.
• Purification Reagents: Optimized for total RNA recovery.
• MiraCol™ Purification Columns: High-yield RNA recovery with contamination prevention.
• Microcentrifuge Tubes: Ready-to-use for RNA purification workflows.
• User Guide and Protocols: Detailed instructions for RNA isolation from various sample types.

Competitive Advantages

1. Small Sample Efficiency:
   • Outperforms other RNA isolation kits in recovering RNA from minute or RNA-poor samples.
2. Preserves mRNA Abundance Classes:
   • Maintains the integrity of low-abundance transcripts, ideal for gene expression studies.
3. Ready-to-Use RNA:
   • RNA is immediately suitable for downstream applications without further processing.
4. Integrated Workflow:
   • Compatible with RiboAmp™ RNA Amplification Kit for a seamless gene expression pipeline.

Limitations

• Not recommended for high-throughput workflows.
• Unsuitable for whole blood, plant, or fungal samples.

For Research Use Only

The Arcturus™ PicoPure™ RNA Isolation Kit is intended for research purposes only and is not suitable for diagnostic or clinical procedures.

Competitor Products

Suggested Pairing

• RiboAmp™ RNA Amplification Kit: For reliable gene expression amplification.
• ArcturusXT™ LCM Instrument: Compatible for Laser Capture Microdissection samples.