Appolo Sci. Chicken egg white lysozyme

The Apollo Scientific Lysozyme from Chicken Egg White (BIL1028) is a highly purified enzyme renowned for its efficiency in breaking down bacterial cell walls. Extracted from chicken egg white, this enzyme is ideal for a variety of molecular biology, microbiology, and biochemistry applications. Its ability to hydrolyze peptidoglycan makes it indispensable in workflows requiring bacterial lysis and protoplast generation.

Key Features:

1. High Efficiency:
   • Provides precise and effective lysis of bacterial cell walls, particularly Gram-positive bacteria, which are rich in peptidoglycan.
2. Broad Applicability:
   • Suitable for genomic DNA extraction, RNA isolation, and preparing bacterial spheroplasts.
   • Used in enzymatic research, bacterial cell wall studies, and structural analyses.
3. Versatile pH Range:
   • Active across a broad pH range, maintaining optimal enzymatic activity under varying experimental conditions.
4. Stability and Purity:
   • Supplied in a stable, purified form for consistent performance.
   • Manufactured under stringent quality controls to ensure reliability and reproducibility.
5. Lyophilized Form:
   • Provided as a lyophilized powder, ensuring long shelf life and ease of storage.

Applications:

1. Molecular Biology:
   • Facilitates the isolation of high-quality genomic DNA and RNA from bacterial samples.
   • Prepares spheroplasts for DNA or protein extraction.
2. Microbiology:
   • Breaks down bacterial peptidoglycan for cell wall studies.
   • Investigates the structural components of bacterial envelopes.
3. Protein and Enzyme Research:
   • Analyzes enzymatic activity and substrate interactions.
   • Useful in studies involving peptidoglycan-binding enzymes.
4. Bacterial Lysis:
   • A gentle yet effective method for lysing bacterial cells without harsh chemicals.
   • Enables efficient recovery of intracellular biomolecules.

Mechanism of Action:

• Lysozyme hydrolyzes β(1→4) linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine, the primary components of peptidoglycan.
• Particularly effective against Gram-positive bacteria due to their high peptidoglycan content.
• Gram-negative bacteria can also be lysed in the presence of agents like EDTA, which disrupt the outer membrane.

Specifications:

Advantages:

1. Gentle and Effective:
   • Avoids harsh chemical treatments, preserving the integrity of extracted biomolecules.
2. Versatile Use:
   • Works in a variety of workflows, from molecular biology to microbiological research.
3. Long Shelf Life:
   • Lyophilized format ensures stability and prolonged usability under proper storage conditions.
4. Reliable Performance:
   • Consistent and reproducible results in both laboratory and industrial applications.

Usage Instructions:

1. Reconstitute the lyophilized powder in sterile buffer according to the experimental needs.
2. For bacterial lysis, add an appropriate concentration of lysozyme to the sample.
3. Incubate at the recommended temperature and pH to allow enzymatic action.
4. Process the sample further for DNA, RNA, or protein isolation.

Applications in Key Research Areas:

• Cell-Based Assays: Lysis of bacterial cells for intracellular studies.
• Genomics: Efficient extraction of genomic materials for sequencing or analysis.
• Proteomics: Preparation of bacterial spheroplasts for protein isolation.

The Apollo Sci. Lysozyme from Chicken Egg White (BIL1028) is a reliable, high-performance enzyme suited for a variety of research needs. Its efficiency in cell lysis, coupled with ease of use, makes it a preferred choice for laboratories worldwide.