Apollo Sci. Kanamycin sulphate, 800 micrograms per mg

Kanamycin sulphate is a broad-spectrum aminoglycoside antibiotic widely used in molecular biology, microbiology, and plant tissue culture. This compound inhibits protein synthesis by binding to the 30S ribosomal subunit, making it effective against Gram-negative and some Gram-positive bacteria. It serves as a selective agent in genetic engineering and molecular cloning applications involving the NPT II (APH3') resistance gene.

Product Features:

1. High Purity:
   • Approximately 800 micrograms of Kanamycin per milligram ensures effective antibacterial activity.
   • Free from contaminants, supporting consistent experimental outcomes.
2. Cell Culture Tested:
   • Suitable for use in sensitive cell culture applications, ensuring compatibility with living systems.
3. Broad Applications:
   • Molecular biology: Used in the selection of transformed cells carrying kanamycin resistance genes.
   • Plant biotechnology: Commonly employed to incorporate the NPT II (APH3') gene in plant tissue culture for selection.
   • Microbial culture: Ensures the growth of kanamycin-resistant bacterial strains while inhibiting susceptible ones.
4. Stable Formulation:
   • Molecular Formula: C₁₈H₃₈N₄O₁₅S
   • Molecular Weight: 582.6 g/mol
5. Storage:
   • Recommended storage at refrigerator temperature (2–8°C) to maintain stability and efficacy.

Use Cases:

1. Molecular Cloning:
   • Essential in plasmid-based cloning to maintain selective pressure and propagate bacteria with kanamycin resistance genes.
2. Plant Tissue Culture:
   • Used in genetic engineering of plants, enabling selection of transformed cells expressing the NPT II gene.
3. Antibiotic Susceptibility Testing:
   • Applied to evaluate bacterial resistance to kanamycin in diagnostic and research labs.
4. Cell Line Maintenance:
   • Supports the propagation of cell lines engineered with kanamycin-resistance markers.
5. Transgenic Research:
   • Ensures selective growth of genetically modified organisms in biotechnological studies.

Specifications:

Safety Notes:

• Follow proper laboratory safety protocols while handling.
• Use personal protective equipment (PPE) to avoid skin and eye contact.
• Dispose of according to local regulatory guidelines.

These attributes make Apollo Sci. Kanamycin Sulphate an ideal choice for researchers requiring reliable and potent antibiotic selection in molecular and cell biology workflows.