Apollo Sci. β-(N-Morpholino)ethanesulphonic acid (MES) ≥99.5%, Ultrapure

High-Purity Zwitterionic Buffer for Precise Biochemical and Formulation Research

β-(N-Morpholino)ethanesulphonic acid (MES) is a Good’s buffer widely used in biological, biochemical, and pharmaceutical applications due to its excellent buffering capacity in the pH range of 5.5 to 6.5. Supplied in ultrapure grade (≥99.5%), the Apollo Scientific MES is ideal for high-precision experiments requiring low UV absorbance and minimal contaminant interference. This product is tested for absorbance <0.05 at 260 nm (1.0 M in H₂O), ensuring compatibility with spectrophotometric and UV-based assays.

Its zwitterionic nature allows MES to maintain buffering capacity without introducing significant ionic strength changes, making it suitable for sensitive enzyme assays, protein stability studies, electrophoresis systems, and formulation development.

Key Features & Benefits

✅ ≥99.5% Ultrapure Grade – Minimizes contaminants in analytical and biochemical workflows

✅ Precise Buffering Range (pKa ~6.1) – Ideal for biological systems with optimal buffering between pH 5.5–6.5

✅ Low UV Absorbance – <0.05 at 260 nm for compatibility with nucleic acid/protein quantification and chromatography

✅ Zwitterionic Stability – Non-reactive, non-chelating, and stable under physiological conditions

✅ Easy Reconstitution & Storage – Forms stable stock solutions for up to 2 weeks at 4°C

Applications

• Protein Stability & Preformulation Studies – Used in the stabilization and solubility evaluation of therapeutic proteins
• Electrophoresis & Chromatography Buffers – Applied in capillary electrophoresis and separation of charged analytes
• Enzyme Assays & Biochemical Reactions – Maintains pH in sensitive biological assays
• Plant Physiology & Ripening Studies – Employed in studies examining polyphenols and metabolic activity (e.g., chlorogenic acid in apples)
• Pharmaceutical Research – Suitable for formulation design and solubility-permeability evaluations
• Neurotoxic Agent Detection – Used in microchip-based separation of organophosphate compounds

Supporting Literature Examples

• Sahni et al. (2017) – Used MES for preformulation and stabilization of acrylodan-labeled glucose-binding protein (SM4-AC)
• Xi et al. (2016) – Employed MES in ripening regulation assays involving chlorogenic acid in apples
• Beig et al. (2013) – Applied MES in solubility-permeability studies of nifedipine with cyclodextrins
• Wang et al. (2001) – Integrated MES buffer in capillary electrophoresis microchips for organophosphate separation

Technical Specifications

Reconstitution & Storage Guidelines

• Reconstitution: Dissolve in distilled water to prepare buffer solutions. Adjust pH using HCl or NaOH as needed.
• Storage: Store the powder and reconstituted stock at 2–8°C. Avoid repeated freeze-thaw cycles.
• Shelf Life: Stable for 2 weeks post-reconstitution. Protect from light and contamination.

Conclusion

Apollo Scientific’s Ultrapure MES Buffer is a reliable, high-purity reagent essential for pH-sensitive biochemical experiments. Its low UV absorbance, zwitterionic nature, and strict purity specifications make it an ideal choice for researchers conducting protein assays, formulation design, and sensitive enzymatic reactions across a wide range of life science applications.