Gibco™ LV-MAX™ Transfection Kit

The Gibco™ LV-MAX™ Transfection Kit is an essential component of the LV-MAX™ Lentiviral Production System, designed for the high-efficiency transient transfection of Gibco Viral Production Cells (high-density, suspension-adapted HEK 293-derived cells). This kit contains a cationic lipid-based transfection reagent, a lentiviral production supplement, and an LV enhancer, all formulated to maximize lentiviral vector production while maintaining cell viability.

With its chemically defined, serum-free, and animal-origin-free formulation, this kit ensures consistent, scalable, and high-yield lentivirus production, achieving titers of up to 1 × 10⁸ TU/mL (unconcentrated LVVs-GFP).

Key Features & Benefits

1. High-Efficiency Lentiviral Production

• Optimized for high-density HEK 293 suspension cell transfection.
• Achieves lentiviral vector titers of up to 1 × 10⁸ TU/mL.
• Delivers higher yields than conventional transfection methods (e.g., PEI-based transfection).

2. Reproducible & Scalable Performance

• Designed for scalable transfection in 96-well plates to 2-L bioreactors.
• Provides high transfection efficiency with minimal toxicity.
• Ensures batch-to-batch consistency for reliable virus production.

3. Serum-Free, Chemically Defined & Animal-Origin-Free

• No animal-derived components, ensuring regulatory compliance.
• Reduces batch variability and potential immunogenic risks.
• Supports high-density cell growth and transfection efficiency.

4. Easy-to-Use & Optimized for Lentivirus Production

• Simplified transfection protocols compatible with transient production workflows.
• Works synergistically with LV-MAX™ Production Medium to enhance viral yield.
• Eliminates the need for additional supplements or optimization steps.

Kit Components & Storage

• Sufficient for 1 L of lentivirus production.
• Do not freeze to maintain reagent stability.

Specifications

Available Product Variant

Applications

1. Lentiviral Vector Production for Gene Therapy & Research

• Optimized for high-yield lentivirus generation in HEK 293-derived Viral Production Cells.
• Superior to PEI-based transfection in terms of titer yield and reproducibility.

2. Cell & Gene Therapy Development

• Ideal for CAR-T cell engineering and gene delivery applications.
• Supports large-scale transient lentivirus production for biotherapeutics.

3. Bioprocessing & Large-Scale Virus Production

• Compatible with 96-well plates to 2-L bioreactors for scalable manufacturing.
• Designed for consistent and reproducible high-density viral transfection.

Storage & Handling

• Store at 2–8°C, protecting reagents from direct light exposure.
• Do not freeze, as freezing may alter reagent stability.
• Maintain aseptic conditions during handling to prevent contamination.

Ordering Information

The Gibco™ LV-MAX™ Transfection Kit is an advanced, high-efficiency transfection reagent system designed to maximize lentiviral vector production in suspension HEK 293-derived Viral Production Cells. It provides reproducible, scalable, and high-yield transfection results while ensuring serum-free, chemically defined, and animal-origin-free formulations. This kit is an essential component for gene therapy research, viral vector manufacturing, and bioprocessing applications.