Liofilchem Bacitracin BA 10 IU disc

The Liofilchem™ Bacitracin BA 10 IU Discs are antibiotic discs containing 10 IU (International Units) of bacitracin. They are used in antimicrobial susceptibility testing (AST) to determine the sensitivity or resistance of bacterial isolates to bacitracin. These discs are widely utilized in clinical microbiology for identification and differentiation of bacterial species, particularly Streptococcus spp., and for quality control in microbiological testing.

Key Features

1. Precision and Standardization:
   • Each disc contains a standardized amount of 10 IU of bacitracin, ensuring reliable and reproducible results in AST.
2. High-Quality Materials:
   • Manufactured using high-grade filter paper for uniform diffusion of the antibiotic.
3. Compatible with CLSI and EUCAST Standards:
   • Designed to comply with international guidelines for susceptibility testing.
4. User-Friendly Packaging:
   • Supplied in packs of 5 cartridges, each containing 50 discs, ideal for medium- to high-throughput laboratories.
5. Wide Range of Applications:
   • Suitable for differentiation, sensitivity testing, and quality control procedures.

Specifications

Use Cases

1. Differentiation of Bacterial Species:
   • Used to differentiate beta-hemolytic Group A Streptococcus (Streptococcus pyogenes) from other streptococcal species based on susceptibility to bacitracin.
2. Antimicrobial Susceptibility Testing (AST):
   • Determines the sensitivity or resistance of bacterial isolates to bacitracin, aiding in the selection of appropriate antibiotic therapy.
3. Quality Control:
   • Used in laboratory quality control procedures to ensure the performance of susceptibility testing systems.
4. Research Applications:
   • Studies on bacitracin resistance mechanisms and bacterial response to bacitracin.

Procedure for Use

1. Prepare a bacterial suspension equivalent to 0.5 McFarland standard.
2. Spread the suspension evenly across the surface of a Mueller-Hinton Agar plate using a sterile swab.
3. Place the Bacitracin BA 10 IU disc onto the inoculated agar surface using sterile forceps or an automated disc dispenser.
4. Incubate the plate at the recommended temperature (typically 35°C) for 18-24 hours.
5. Measure the zone of inhibition around the disc to determine susceptibility or resistance.

Advantages

• Reliable and Accurate: Standardized disc content ensures consistent performance in AST.
• High Sensitivity: Effective for identifying beta-hemolytic Group A Streptococcus.
• Convenient Packaging: 5x50 disc format ideal for efficient workflow in clinical and research laboratories.
• Standards-Compliant: Compatible with CLSI and EUCAST guidelines for susceptibility testing.

Packaging

• Quantity: 5 cartridges, each containing 50 discs (250 discs total).
• Storage Conditions: Store at 2°C to 8°C to maintain stability and effectiveness.

Why Choose Liofilchem Bacitracin BA 10 IU Discs?

• Trusted for bacterial differentiation and antimicrobial susceptibility testing.
• Provides consistent and reliable results across various microbiological applications.
• Manufactured to meet international standards for quality and performance.