Liofilchem® Norfloxacin NOR Antimicrobial Susceptibility Discs, 10 µg

Liofilchem® Norfloxacin NOR Antimicrobial Susceptibility Discs (10 µg) are standardized, high-quality paper discs impregnated with 10 micrograms of Norfloxacin, a second-generation fluoroquinolone antibiotic. These discs are designed for in vitro antimicrobial susceptibility testing (AST) using the Kirby-Bauer disk diffusion method. Norfloxacin is primarily active against Gram-negative organisms, especially Escherichia coli, Proteus spp., Klebsiella spp., Pseudomonas aeruginosa, and other urinary tract pathogens.

Manufactured under ISO 9001 and ISO 13485 quality systems, Liofilchem® Norfloxacin discs provide accurate, reproducible results for clinical, pharmaceutical, veterinary, and research microbiology laboratories.

Key Features

• Standardized potency: Each disc contains 10 µg of Norfloxacin, ensuring consistent zone of inhibition.
• Broad-spectrum activity: Effective against a variety of Gram-negative bacilli and some Gram-positive cocci.
• CLSI/EUCAST compatible: Suitable for interpretation using current antimicrobial breakpoint standards.
• Reliable and stable: Discs are packed in sealed cartridges or canisters with desiccants to prevent moisture and ensure long shelf life.
• Clear labeling: Color-coded and pre-labeled for easy identification and traceability in laboratory workflows.

Applications and Use Cases

1. Clinical Microbiology

• Susceptibility testing of urinary tract pathogens like E. coli, Proteus mirabilis, Klebsiella pneumoniae.
• Differentiation of quinolone susceptibility in Gram-negative isolates from wound, blood, and respiratory cultures.

2. Pharmaceutical Quality Control

• MIC screening and validation of Norfloxacin-based formulations and generics.
• Support in regulatory submissions and antibiotic potency testing.

3. Veterinary Diagnostics

• AST of enteric and urinary tract pathogens in livestock and companion animals.

4. Antibiotic Resistance Monitoring

• Surveillance of Norfloxacin resistance patterns in hospitals, environmental labs, and foodborne pathogen studies.

Use Instructions

1. Medium: Mueller-Hinton Agar (MHA) is recommended for standard testing.
2. Inoculum: Prepare a 0.5 McFarland standard suspension of the test organism.
3. Inoculation: Swab the inoculum evenly over the surface of the agar plate.
4. Disc application: Place the 10 µg Norfloxacin disc onto the agar surface using sterile forceps or a disc dispenser.
5. Incubation: 35 ± 2 °C for 16–18 hours in ambient air.
6. Interpretation: Measure the diameter of the inhibition zone (in mm) and compare to CLSI or EUCAST interpretive criteria for Norfloxacin.

Technical Specifications

Quality Control

Each batch is tested using recognized control strains to verify performance:

Liofilchem® Norfloxacin NOR Discs (10 µg) are a dependable and validated solution for fluoroquinolone susceptibility testing in Gram-negative pathogens. With their broad application in clinical, veterinary, and pharmaceutical microbiology, these discs support effective antibiotic therapy decisions and resistance tracking. Their precision and compliance with international standards make them an essential component in AST workflows.