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Specifications:
| Application | Microbiology | ||
| Storage Temperature | Room Temperature | ||
| Product Type | Culture Medium | Forms | Powder |
| Product Brand | Liofilchem | ||
| Product Grade | Microbiology grade | ||
Liofilchem® Motility Test Agar is a dehydrated culture medium formulated for the detection of motility in gram-negative enteric bacilli. It complies with the UNI EN ISO 11290-1:2005 standard and is widely used in clinical and food microbiology laboratories. This low-concentration agar formulation enables visible detection of motile bacterial species via the diffusion of growth from the stab line in tube inoculations.
Key Features
- Standardized Composition: Contains casein peptone and meat extract to provide essential nutrients, with a reduced agar content (3.5 g/L) to support motility detection.
- Validated Method: Developed according to ISO 11290-1:2005 guidelines for routine testing of gram-negative enteric bacteria.
- Clear Visualization: Low agar concentration creates a semi-solid matrix allowing motile bacteria to spread while non-motile bacteria remain along the stab line.
- Versatile Packaging: Available in 100 g and 500 g plastic bottles for flexible lab-scale preparation.
- Extended Shelf Life: Up to 4 years when stored under recommended conditions.
Typical Composition (per liter)
| Component | Concentration |
|---|---|
| Casein Peptone | 20.0 g/L |
| Meat Extract | 6.1 g/L |
| Agar | 3.5 g/L |
| Final pH | 7.2 ± 0.2 |
Applications and Use Cases
1. Clinical Microbiology
- Differentiation of motile and non-motile Enterobacteriaceae, e.g., Escherichia coli (motile) vs Klebsiella pneumoniae (non-motile).
2. Food and Water Testing
- Detection of motility as part of Listeria spp. differentiation workflow, especially in food safety labs.
3. Educational Use
- Demonstrating microbial motility in academic settings and microbiology training programs.
Use Instructions
Preparation:
- Suspend 29.6 g of powder in 1 L of distilled or deionized water.
- Heat to boiling with gentle shaking until completely dissolved.
- Dispense into final containers.
- Sterilize by autoclaving at 121°C for 15 minutes.
Inoculation:
- Inoculate by vertically stabbing a pure culture into the medium to more than half the depth of the tube.
Incubation:
- Primary: 36 ± 1°C for 24–48 hours in aerobic conditions.
- Optional: Reincubate negative tubes at 25 ± 2°C for up to 5 days.
Interpretation:
- Motile organisms: Show diffuse or hazy growth radiating from the stab line.
- Non-motile organisms: Growth confined to the stab line only.
Performance & Quality Control
| Test Organism | Growth | Motility |
|---|---|---|
| Enterobacter aerogenes ATCC 13048 | Good | + |
| Escherichia coli ATCC 25922 | Good | + |
| Klebsiella pneumoniae ATCC 13883 | Good | – |
- Sterility Testing: 7 days at 22 ± 1°C and 36 ± 1°C
- Productivity Testing: Inoculum 10–100 CFU/mL, incubation 18–24 h at 36 ± 1°C
Storage and Shelf Life
- Storage Conditions (Powder): 10–30°C in a dry environment, sealed container.
- Storage Conditions (Prepared Medium): 2–8°C
- Shelf Life: 4 years from date of manufacture
Product Information
| Reference | Content | Packaging |
|---|---|---|
| 610132 | 500 g | Plastic bottle |
| 620132 | 100 g | Plastic bottle |
Precautions and Waste Disposal
- For in vitro diagnostic use only by trained personnel.
- No hazardous substances in concentrations exceeding regulatory limits.
- Dispose of according to national and local biosafety and chemical regulations.
Liofilchem® Motility Test Agar is a reliable and easy-to-use medium for determining microbial motility, especially in gram-negative enteric bacteria. Its formulation ensures optimal clarity of results, supports microbial growth, and adheres to international standards. Whether used in clinical, food, or academic laboratories, it is an essential tool for bacterial characterization and quality control.