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BD™ CBA Human Th1/Th2/Th17 Cytokine Kit, 80 Tests
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Specifications:
| Application | Flow Cytometry | ||
| Storage Temperature | 2-8°C | ||
| Product Type | Assay Kits | Forms | Kit with Various components |
| Product Brand | BD Biosciences | ||
| Product Grade | Molecular Biology | ||
Compact, High-Performance Multiplex Assay for Comprehensive T Helper Cell Immune Profiling
The BD™ CBA Human Th1/Th2/Th17 Cytokine Kit (Catalog No. 560484) enables simultaneous quantification of seven key cytokines associated with Th1, Th2, and Th17 immune responses. This flow cytometry–based multiplex assay detects IL-2, IL-4, IL-6, IL-10, IL-17A, TNF, and IFN-γ in a single human sample, using distinct fluorescent bead populations and a PE-conjugated detection reagent. Ideal for translational research, immunology, and biomarker discovery, this kit supports high-throughput profiling of immune status and inflammation.
All reagents are validated for RUO (Research Use Only) and optimized for use with serum, plasma, and cell culture supernatants. With BD’s gold-standard bead array technology, the kit provides superior data quality, sensitivity, and sample efficiency.
Key Features
Expanded Multiplexing: Measures 7 cytokines representing Th1 (IL-2, IFN-γ, TNF), Th2 (IL-4, IL-10), and Th17 (IL-17A) responses
Bead-Based Assay: Flow cytometry–compatible design using distinguishable fluorescent bead populations
Validated Sample Types: Optimized for human serum, plasma, and tissue culture supernatants
High Sensitivity: Detects pg/mL levels of cytokines with minimal sample input
PE Detection Chemistry: For accurate quantification of cytokine levels across samples
Comprehensive Reagents: Includes capture beads, PE detection reagent, standards, controls, buffers, and enhancement reagents
RUO-Labeled: Designed for research purposes only; not for diagnostic use
Applications
- T Helper Cell Subset Profiling (Th1/Th2/Th17)
- Cytokine Response Analysis in Infection or Autoimmunity
- Vaccine and Immunotherapy Research
- Multiplex Biomarker Discovery
- Longitudinal Monitoring of Cytokine Expression
Technical Specifications
| Attribute | Specification |
|---|---|
| Product Name | BD™ CBA Human Th1/Th2/Th17 Cytokine Kit |
| Catalog Number | 560484 |
| Detection Method | Flow cytometry with PE detection reagent |
| Cytokines Measured | IL-2, IL-4, IL-6, IL-10, IL-17A, TNF, IFN-γ |
| Sample Types | Human serum, EDTA plasma, culture supernatants |
| Kit Format | Multiplex bead-based assay, 80 tests |
| Reactivity | Human (QC Tested) |
| Regulatory Status | RUO – Research Use Only |
| Storage Conditions | 2–8 °C (Do not freeze) |
| Brand | BD™ Cytometric Bead Array (CBA) |
| RRID | AB_2869353 |
Kit Components
| Component | Part Number | Quantity |
|---|---|---|
| IL-2 Capture Beads | 51-9005736 | 80 Tests |
| IL-4 Capture Beads | 51-9005715 | 1 Each |
| IL-6 Capture Beads | 51-9005738 | 80 Tests |
| IL-10 Capture Beads | 51-9005740 | 80 Tests |
| TNF Capture Beads | 51-9005724 | 1 Each |
| IFN-γ Capture Beads | 51-9005727 | 1 Each |
| IL-17A Capture Beads | 51-9006289 | 100 Tests |
| PE Detection Reagent | 51-9006291 | 1 Vial |
| Cytokine Standards (lyophilized) | 51-9006249 | 2 Vials |
| Cytometer Setup Beads | 51-9005731 | 1 Each |
| PE Positive Control Detector | 51-9005732 | 1 Each |
| FITC Positive Control Detector | 51-9005734 | 1 Each |
| Wash Buffer | 51-2431KC | 1 Bottle |
| Assay Diluent | 51-2432KC | 1 Bottle |
| Serum Enhancement Buffer | 51-9002150 | 1 Bottle |
The BD™ Human Th1/Th2/Th17 CBA Kit provides a comprehensive, efficient, and sensitive method for multiplex cytokine profiling. With seven critical analytes covered, this flow cytometry–based platform empowers researchers to deeply understand immune regulation in health and disease, all from a single sample.