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Specifications:
| Application | Cell culture | ||
| Storage Temperature | 2-8°C | ||
| Product Type | Supplement | Forms | Liquid |
| Product Brand | Thermo Fisher Scientific™, Gibco | ||
| Product Grade | Cell Culture | ||
Buffered Cell Culture Medium for Mammalian Cell Lines – Without Glutamine
Gibco™ MEM (Minimum Essential Medium), with HEPES and Phenol Red, but without L-glutamine, is a widely used basal medium suitable for the growth and maintenance of a broad range of suspension and adherent mammalian cells, including HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, fibroblasts, and primary rat astrocytes. Modified with HEPES buffer for enhanced pH stability in non-CO₂ environments, this formulation is ideal for use in research and manufacturing workflows requiring precise environmental control.
Key Features
- HEPES Buffering System: Maintains pH stability in open or non-CO₂ incubator systems
- No L-Glutamine: Allows flexibility to supplement with glutamine or GlutaMAX™ as needed
- Sterile-Filtered: Ready-to-use with standard aseptic workflows
- Serum Supplementation Required: Typically used with 10% FBS
- Earle’s Balanced Salts: For use with 5–10% CO₂ incubation
- Animal-Origin Free: Manufactured without animal-derived components
- Manufactured under cGMP conditions and ISO 13485 certification
Applications
- Routine Mammalian Cell Culture
- Viral Propagation Studies
- Transfection and Protein Expression
- Research-Grade Cell Maintenance and Expansion
Specifications
| Attribute | Details |
|---|---|
| Product Type | Minimum Essential Medium (MEM) |
| Buffer System | HEPES (Good’s Buffer), Sodium Bicarbonate |
| Cell Lines Supported | HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, Fibroblasts |
| Primary Cell Type | Rat Astrocytes |
| Form | Liquid |
| Glutamine | Not included |
| Glucose | Low glucose formulation |
| Phenol Red | Included |
| Pyruvate | Not included |
| Concentration | 1X |
| Serum Level | Standard serum supplementation required |
| Sterility | Sterile-filtered |
| Animal Origin | Animal origin-free |
| cGMP Compliance | Yes, ISO 13485-certified facility |
| Storage Temperature | 2–8°C; protect from light |
| Shipping Condition | Ambient |
| Shelf Life | 12 months from date of manufacture |
| Unit Size | 500 mL |
Gibco™ MEM (HEPES, no glutamine) offers a flexible, reliable platform for cell culture research, especially when independent glutamine supplementation or HEPES buffering is preferred. With high-quality cGMP production and compatibility across numerous cell lines, it supports consistent results in routine and advanced cell culture applications.