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Invitrogen™ Plant DNAzol™ Reagent is a single-step, ready-to-use organic extraction reagent formulated for the rapid and efficient isolation of high-quality genomic DNA from a wide range of plant tissues, including leaves, seeds, stems, roots, and callus. Utilizing a guanidine-detergent lysing solution, this reagent hydrolyzes RNA during extraction and selectively precipitates DNA, eliminating the need for RNase treatment in most cases.
Designed to streamline plant DNA extraction workflows, Plant DNAzol™ offers a faster and simpler alternative to traditional CTAB-based protocols, while maintaining comparable DNA yield and quality.
Key Features and Benefits
- Broad Compatibility: Effective on a wide range of plant materials (leaf, seed, stem, root, callus).
- One-Hour Protocol: Complete DNA isolation in approximately 60–70 minutes.
- Efficient RNA Removal: Hydrolyzes RNA without the need for RNase A.
- No Phenol Required: Eliminates phenol use in most tissue types.
- Comparable to CTAB: Matches traditional methods in yield and quality with fewer steps and reagents.
- Versatile Use: DNA is suitable for PCR, cloning, sequencing, Southern blotting, AFLP, and RFLP.
Workflow Overview
- Pulverize or homogenize plant tissue.
- Lyse with Plant DNAzol™ Reagent.
- Centrifuge to remove debris.
- Precipitate DNA with ethanol.
- Solubilize DNA in TE buffer (pH 8.0).
- Use directly in downstream applications.
Technical Specifications
| Parameter | Specification |
|---|---|
| Final Product Type | Genomic DNA |
| Isolation Technology | Organic Extraction (Guanidine-detergent lysis) |
| Yield | Up to 300 µg DNA per 0.1 g plant material |
| Elution Volume | ~70 µL |
| Sample Type | Plant tissue (leaf, seed, root, stem, callus) |
| Starting Material | 0.1 g plant tissue |
| Test Time | ~60 minutes |
| High-throughput Compatibility | Manual use only |
| Applications | PCR, cloning, sequencing, AFLP, RFLP, Southern blotting |
| Shipping Conditions | Room temperature |
| Storage Conditions | Room temperature |
| Unit Size | 100 mL bottle |
Contents & Storage
- Contents: 100 mL Plant DNAzol™ Reagent
- Storage: Store at room temperature for routine use
Invitrogen™ Plant DNAzol™ Reagent offers a fast, reliable, and simplified method for extracting high-quality genomic DNA from diverse plant materials. With built-in RNA hydrolysis and compatibility with major downstream applications, it’s an ideal solution for researchers seeking efficient nucleic acid purification without the complexity and hazards of traditional protocols. Suitable for molecular biology, plant genetics, and agricultural research labs.
User Guide
Invitrogen™ Plant DNAzol™ Reagent is a single-step, ready-to-use organic extraction reagent formulated for the rapid and efficient isolation of high-quality genomic DNA from a wide range of plant tissues, including leaves, seeds, stems, roots, and callus. Utilizing a guanidine-detergent lysing solution, this reagent hydrolyzes RNA during extraction and selectively precipitates DNA, eliminating the need for RNase treatment in most cases.
Designed to streamline plant DNA extraction workflows, Plant DNAzol™ offers a faster and simpler alternative to traditional CTAB-based protocols, while maintaining comparable DNA yield and quality.
Key Features and Benefits
- Broad Compatibility: Effective on a wide range of plant materials (leaf, seed, stem, root, callus).
- One-Hour Protocol: Complete DNA isolation in approximately 60–70 minutes.
- Efficient RNA Removal: Hydrolyzes RNA without the need for RNase A.
- No Phenol Required: Eliminates phenol use in most tissue types.
- Comparable to CTAB: Matches traditional methods in yield and quality with fewer steps and reagents.
- Versatile Use: DNA is suitable for PCR, cloning, sequencing, Southern blotting, AFLP, and RFLP.
Workflow Overview
- Pulverize or homogenize plant tissue.
- Lyse with Plant DNAzol™ Reagent.
- Centrifuge to remove debris.
- Precipitate DNA with ethanol.
- Solubilize DNA in TE buffer (pH 8.0).
- Use directly in downstream applications.
Technical Specifications
| Parameter | Specification |
|---|---|
| Final Product Type | Genomic DNA |
| Isolation Technology | Organic Extraction (Guanidine-detergent lysis) |
| Yield | Up to 300 µg DNA per 0.1 g plant material |
| Elution Volume | ~70 µL |
| Sample Type | Plant tissue (leaf, seed, root, stem, callus) |
| Starting Material | 0.1 g plant tissue |
| Test Time | ~60 minutes |
| High-throughput Compatibility | Manual use only |
| Applications | PCR, cloning, sequencing, AFLP, RFLP, Southern blotting |
| Shipping Conditions | Room temperature |
| Storage Conditions | Room temperature |
| Unit Size | 100 mL bottle |
Contents & Storage
- Contents: 100 mL Plant DNAzol™ Reagent
- Storage: Store at room temperature for routine use
Invitrogen™ Plant DNAzol™ Reagent offers a fast, reliable, and simplified method for extracting high-quality genomic DNA from diverse plant materials. With built-in RNA hydrolysis and compatibility with major downstream applications, it’s an ideal solution for researchers seeking efficient nucleic acid purification without the complexity and hazards of traditional protocols. Suitable for molecular biology, plant genetics, and agricultural research labs.