Invitrogen™ eBioscience™ IC Fixation Buffer, 125mL

The Invitrogen™ eBioscience™ Intracellular (IC) Fixation Buffer is a specialized reagent designed for fixing cells prior to permeabilization when performing intracellular staining. It is ideal for analyzing antigens located within the cytoplasm and secretory pathways, including cytokines, chemokines, and growth factors. This fixation buffer is compatible with fluorochrome-conjugated antibodies used for surface staining and ensures reliable results in intracellular staining workflows, particularly when combined with the Permeabilization Buffer (Cat. 00-8333).

Key Features:

1. High Compatibility:
   • Suitable for intracellular staining of cytokines, chemokines, and other intracellular proteins.
   • Compatible with surface staining fluorochrome-conjugated antibodies, including tandem dyes.
2. Short-Term Storage:
   • Allows for short-term storage of fluorochrome-stained cells for up to 3 days at 2–8°C (stored in the dark).
3. Optimized for Flow Cytometry:
   • Specifically designed for intracellular staining followed by flow cytometric analysis.
4. Easy to Use:
   • Ready-to-use formulation simplifies workflows and ensures consistent results.

Specifications:

Applications:

1. Intracellular Staining:
   • Fixes cells for the detection of intracellular antigens like cytokines, chemokines, and growth factors.
2. Flow Cytometry:
   • Used in combination with permeabilization reagents to enable intracellular and surface staining for cytometric analysis.
3. Cell Preparation:
   • Acts as a fixation buffer to preserve cell integrity and antigen localization for subsequent staining.

Key Benefits:

1. Reliable Cell Fixation:
   • Stabilizes intracellular antigens for accurate staining and analysis.
2. Flexibility:
   • Works seamlessly with fluorochrome-conjugated antibodies for multicolor flow cytometry.
3. Convenient Storage:
   • Allows short-term storage of fixed and stained cells, reducing workflow pressure.
4. Validated for Research:
   • Provides consistent, reproducible results for intracellular staining experiments.

Storage and Handling:

• Storage Temperature: Store at 2–8°C in the dark.
• Do not freeze the buffer to maintain its functionality.

Research Use Only:

This product is intended for research purposes only and is not suitable for diagnostic or therapeutic use.