Thermo Scientific™ Dansyl chloride 98%

Fluorogenic Reagent for N-Terminal Derivatization in Peptide and Protein Analysis

Thermo Scientific™ Dansyl Chloride (5-(Dimethylamino)naphthalene-1-sulfonyl chloride) is a high-purity fluorogenic reagent widely used in protein chemistry, bioanalysis, and chromatographic derivatization workflows. This 98% grade compound is specifically designed for the fluorescent labeling of amino acids, peptides, and amines through N-terminal derivatization, allowing highly sensitive detection by reverse phase high-performance liquid chromatography (RP-HPLC) and fluorescence spectroscopy.

Its applications span clinical diagnostics, proteomics, enzymology, and structural biology, enabling advanced studies such as protein conformational analysis, fluorescent conjugate formation, and active site characterization.

Key Features & Benefits

• ✅ High Purity (98%) – Ensures consistent and reproducible derivatization
• ✅ Fluorogenic Labeling Agent – Produces stable fluorescent derivatives for sensitive detection
• ✅ Versatile Applications – Suitable for peptides, amino acids, biological fluids, and neural samples
• ✅ RP-HPLC Compatibility – Ideal for fluorescent detection in reversed-phase chromatography
• ✅ Research-Grade Quality – Trusted for high-resolution analytical workflows and biochemical studies

Applications

• Fluorescent N-terminal derivatization of amino acids and peptides
• Detection of derivatized compounds by RP-HPLC or fluorescence detection
• Protein structural studies and conformational change analysis
• Quantification of immobilized enzymes (e.g., alkaline phosphatase)
• Labeling amines in plasma, neural, and other biological samples
• Preparation of fluorescent protein conjugates for imaging or activity assays

Biochemical/Physiological Actions

Dansyl chloride is a sulfonyl chloride derivative that covalently attaches to free amino groups, enabling:

• Tagging of proteins and peptides for visualization
• Derivatization of primary amines in biological matrices
• Studies of protein dynamics, folding, and enzyme active sites

Thermo Scientific™ Dansyl Chloride, 98% is a reliable and high-performance reagent for fluorescent tagging and advanced bioanalytical studies. Its broad utility across proteomics, chromatography, and fluorescence-based assays makes it a critical tool for researchers demanding precision and sensitivity in complex biological systems.