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PageRuler™ Plus Prestained Protein Ladder, 10 to 250 kDa
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Dye-stained proteins in 62.5mM Tris-H3PO4 (pH 7.5 at 25°C), 1mM EDTA, 2% SDS, 10mM DTT, 1mM NaN3 and 33% glycerol.
Specifications:
| Application | Protein Electrophoresis & Western Blotting | ||
| Storage Temperature | -20°C | ||
| Product Type | Protein Gels | Forms | Liquid |
| Product Brand | Thermo Fisher Scientific™ | ||
| Product Grade | Molecular Biology | ||
The Thermo Scientific™ PageRuler™ Plus Prestained Protein Ladder is a high-quality molecular weight marker composed of nine prestained proteins ranging from 10 to 250 kDa. These markers are visually distinguished by three different colors—blue, orange, and green—to simplify protein band identification and gel orientation during SDS-PAGE and Western blotting.
This ladder is supplied in a ready-to-use format—no heating, reducing, or additional buffer preparation is required. It is ideal for tracking protein migration, monitoring membrane transfer, and estimating molecular weights in a variety of electrophoresis systems, including NuPAGE™, Bolt™, and Novex™ gels.
Key Features and Benefits
- Nine prestained proteins in three colors (blue, orange, green)
- Covers broad range: 10 kDa to 250 kDa
- Ready to load – no heating, reducing, or mixing required
- Multi-color banding simplifies molecular weight estimation and gel orientation
- Sharp, consistent bands compatible with SDS-PAGE and Western blot applications
- Fluorescence compatible: NIR (700 nm), RGB (555 nm) for advanced imaging
- Validated across major gel types and transfer systems
Applications
- SDS-PAGE and Tris-Glycine gels
- Western blotting and membrane transfer confirmation
- Accurate molecular weight determination
- Gel orientation during electrophoresis
Technical Specifications
| Attribute | Description |
|---|---|
| Product Name | PageRuler™ Plus Prestained Protein Ladder |
| Catalog Numbers | 26619 (2 × 250 µL), 26620 (10 × 250 µL) |
| Number of Bands | 9 |
| Size Range | 10 to 250 kDa |
| Colors | Blue, Orange, Green |
| Format | Ready-to-load (no preparation needed) |
| Compatibility | SDS-PAGE, Western blot, Bolt™, NuPAGE™, Novex™ gels |
| Detection | Colorimetric, NIR (700 nm), RGB Fluorescence (555 nm) |
| Shipping | Wet or Dry Ice |
| Storage Conditions | –20 °C |
| Storage Buffer | Tris-H₃PO₄ (pH 7.5), SDS, DTT, NaN₃, glycerol |
The PageRuler™ Plus Prestained Protein Ladder offers fast, reliable, and multi-color reference bands for monitoring protein migration and estimating molecular weights in protein gel electrophoresis and Western blotting. Its broad size range, vivid color differentiation, and compatibility with fluorescent detection systems make it an indispensable standard for modern protein analysis workflows.
- Pack Size: 2 x 250 µL 10 x 250 µL