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Gibco™ Geneticin™ Selective Antibiotic (G418 Sulfate), Powder
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Specifications:
| Application | Cell culture | ||
| Storage Temperature | Room Temperature | ||
| Product Type | Antibiotic | Forms | Powder |
| Product Brand | Thermo Fisher Scientific™, Gibco | ||
| Product Grade | Cell Culture | ||
Ribosome-Targeting Antibiotic for Selection of Genetically Engineered Cells
Gibco™ Geneticin™ Selective Antibiotic (G418 Sulfate) is a broad-spectrum aminoglycoside antibiotic produced by Micromonospora rhodorangea. It functions by binding to the 80S and 70S ribosomes in eukaryotic and prokaryotic cells, respectively, thereby inhibiting protein synthesis. Widely used in molecular biology and cell culture, G418 is a reliable agent for selecting and maintaining genetically modified cells that express resistance genes such as APH (3')-I or APH (3')-II.
This product is supplied in powder form and is designed for preparation of aqueous stock solutions for use in bacterial and mammalian cell culture systems.
Key Features and Benefits
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High-Purity Selection Agent
Inhibits protein synthesis in both prokaryotic and eukaryotic cells by targeting ribosomes. -
Effective Selection Tool
Enables stable cell line generation by eliminating non-transfected cells in cultures expressing neomycin resistance genes. -
Flexible Concentration Range
- 100–200 μg/mL for bacteria
- 200–500 μg/mL for most mammalian cells
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Dual-Site cGMP Manufacturing
Manufactured in Grand Island, NY and Scotland, UK under ISO 13485 and FDA-registered facilities for supply chain continuity and regulatory compliance.
Applications
- Stable transfection selection in mammalian cells expressing neomycin resistance
- Selection of genetically engineered bacteria
- Long-term maintenance of transgenic or recombinant cell lines
- Cell line development for protein expression or functional studies
Technical Specifications
| Property | Details |
|---|---|
| Product Name | Geneticin™ (G418 Sulfate), Powder |
| Catalog Numbers | 11811023 (1 g), 11811031 (5 g), 11811098 (25 g) |
| Form | Powder |
| Purity | High-purity, non-sterile |
| Recommended Stock | 10–50 mg/mL in sterile water |
| Working Concentration | 100–200 μg/mL (bacteria), 200–500 μg/mL (mammalian cells) |
| Target Organisms | Eukaryotic and prokaryotic cells |
| Mechanism of Action | Ribosomal binding; inhibition of protein synthesis |
| Resistance Genes | APH(3')-I and APH(3')-II |
| Shelf Life | 24 months from manufacture date |
| Storage Conditions | 15–30°C (ambient); protect from moisture |
| Shipping Condition | Ambient temperature |
| Product Line | Geneticin™ |
| Unit Size | Each (1 g, 5 g, or 25 g) |
| Sterility | Non-sterile |
| Intended Use | For research use only. Not for human or animal use. |
Gibco™ Geneticin™ Selective Antibiotic (G418 Sulfate) is a trusted selection agent for stable cell line generation and maintenance in genetic engineering workflows. Its dual-target activity in eukaryotic and prokaryotic cells, along with robust manufacturing controls and flexible usage across applications, makes it an essential tool in biotechnology and cell-based research.
- Pack Size: 1g 5g 25g