AMPLIRUN® Salmonella DNA Control, 10,000-20,000 copies/µl

The AMPLIRUN® Salmonella DNA Control consists of purified, quantified complete microbial genomes from Salmonella enteritidis or Salmonella typhi. It is designed as a standardized control for molecular amplification techniques such as PCR, qPCR, and other nucleic acid-based assays. By providing a quantified, non-infectious reference material, it improves assay accuracy, validation, and reproducibility in both research and molecular diagnostic development.

Key Features

• Purified complete microbial genome (any sequence can be amplified).
• Quantified DNA concentration: 10,000–20,000 copies/µL, determined by qPCR.
• Lyophilized format for enhanced stability and simplified shipping.
• Non-infectious control for safe laboratory use.
• Supplied with Molecular Biology Grade Water for reconstitution.
• Compatible with all molecular testing platforms (PCR, qPCR, sequencing).
• Sequencing data available to assist probe/primer design.

Ordering Information

Each vial must be reconstituted with 100 µL of DNase/RNase-free Molecular Biology Grade Water (included).

Salmonella typhi Genes/Fragments Sequence data

• AY370864.1
• Z47544.1

Salmonella enteritidis complete genome 

• CP173222

Applications

• Positive control in molecular assays for Salmonella detection.
• Assay validation: sensitivity, specificity, precision, and LOD (limit of detection).
• Absolute quantification: generate standard curves in qPCR.
• Training & quality control: reproducible benchmark for molecular testing workflows.
• Research & development: assay optimization and primer/probe design.
• Alternative to clinical samples: avoids biosafety risks and sample variability.

Clinical & Research Significance

• Salmonella enteritidis and S. typhi are clinically significant pathogens in foodborne illness and typhoid fever, respectively.
• Accurate nucleic acid amplification-based detection of these pathogens is critical for public health surveillance, food safety testing, and research laboratories.
• Using AMPLIRUN® DNA controls ensures consistency across laboratories and eliminates variability that arises from using clinical isolates.

The AMPLIRUN® Salmonella DNA Controls are quantified, lyophilized, and non-infectious genomic DNA standards that improve the reliability and reproducibility of nucleic acid amplification assays. They are ideal for research labs, molecular diagnostic developers, and quality control programs working with Salmonella detection.