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MERCK Chromocult® Coliform Agar ES
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suitable for microbiology
Specifications:
| Application | Microbiology | ||
| Storage Temperature | Ambient | ||
| Product Type | Culture Medium | Forms | Granules |
| Product Brand | MERCK, Millipore | ||
| Product Grade | Microbiology grade | ||
Chromogenic Culture Medium for Simultaneous Detection and Enumeration of E. coli and Coliforms in High-Burden Food Samples
Chromocult® Coliform Agar ES is a selective and differential chromogenic medium designed for the simultaneous detection and enumeration of Escherichia coli and other coliform bacteria in food and feed samples. With enhanced selectivity, this medium is particularly suited for samples with high bacterial bioburden, enabling accurate colony counts and easy differentiation based on distinct chromogenic reactions.
Key Features & Benefits
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Dual chromogenic substrates:
- Salmon-β-D-GAL for total coliforms → salmon to red colonies
- X-β-D-glucuronide for E. coli → blue colonies
- E. coli, which cleaves both substrates → dark violet colonies
- Enhanced selectivity: Inhibits Gram-positive and non-target Gram-negative bacteria using bile salts and propionate.
- Optimized peptone blend and MOPS buffering: Promotes rapid growth and enzymatic activity of target organisms.
- Time-efficient: Enables clear detection and enumeration of target colonies within 24 hours.
Applications
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Detection and enumeration of E. coli and coliforms in:
- Raw ground beef and poultry
- Raw milk and dairy products
- Animal feed and high-load food samples
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Suitable for use in:
- Food microbiology laboratories
- Routine quality control and HACCP compliance
- Veterinary and feed analysis labs
Specifications
| Parameter | Value / Description |
|---|---|
| Product Form | Granular (Dehydrated culture medium) |
| Sterility | Non-sterile |
| Quality Level | 200 |
| pH Range (25 °C) | 6.8–7.2 |
| Solubility | 34.6 g/L |
| Preparation | 34.6 g/L in distilled water, autoclave at 121 °C |
| Storage Temperature | 15–25 °C (room temperature) |
| Target Organisms | E. coli, coliforms |
| Incubation Conditions | 24 hrs, 35 °C, aerobic |
Colony Appearance
| Organism | Colony Color |
|---|---|
| E. coli | Blue (dark violet if both substrates cleaved) |
| Citrobacter freundii, Enterobacter aerogenes | Salmon to red |
| Other flora (e.g., Staphylococcus aureus, Enterococcus faecalis, Bacillus subtilis, Aeromonas hydrophila) | Growth inhibited |
Recovery & Selectivity Data (≥70%)
- Escherichia coli ATCC 11775
- Citrobacter freundii ATCC 8090
- Enterobacter aerogenes ATCC 13048
Inhibited Strains:
- Staphylococcus aureus
- Enterococcus faecalis
- Aeromonas hydrophila
- Bacillus subtilis
Chromocult® Coliform Agar ES offers a highly selective, ISO-relevant solution for the rapid and accurate detection of E. coli and coliforms in complex food and feed matrices. With dual chromogenic indicators, robust selectivity, and reliable colony distinction, this medium enhances workflow efficiency and regulatory compliance in food safety and microbiology laboratories.