Sample Buffer, Laemmli 2× Concentrate, 6mL

Sigma-Aldrich Laemmli Sample Buffer (2× concentrate) is a ready-to-use protein sample preparation buffer designed for SDS-PAGE applications following the Laemmli method. This formulation ensures optimal protein denaturation, reduction, and tracking during electrophoretic separation, enabling high-resolution analysis of protein molecular weight and purity.

Key Features

• SDS-Based Denaturing Buffer – Ensures proteins acquire a uniform negative charge for size-based separation
• Ready-to-Use 2× Concentrate – Mix 1:1 with your protein sample prior to loading
• Complete Sample Preparation – Contains reducing agent, tracking dye, and density enhancer for efficient sample handling
• Optimized for Laemmli SDS-PAGE System – Proven formulation for high-resolution protein electrophoresis

Composition

Biochemical Function

• Denaturation: SDS disrupts protein structure, while 2-mercaptoethanol reduces disulfide bonds
• Charge Equalization: Proteins acquire a consistent negative charge to enable separation by size
• Sample Visibility & Tracking: Bromophenol blue acts as a visual loading and migration marker
• Sample Layering: Glycerol ensures that samples settle efficiently in gel wells

Usage Instructions

• Recommended Usage: Mix 1 volume of sample with 1 volume of 2× Laemmli buffer
• Preparation: Heat the mixture at 95–100°C for 2–5 minutes before loading into the gel

Applications

• SDS-PAGE Analysis – For protein size estimation, purity analysis, and Western blotting
• Biochemical & Molecular Biology Research – Suitable for sample preparation in proteomics workflows
• Education & Training Labs – Standard buffer for protein electrophoresis protocols

Sigma-Aldrich Laemmli 2× Sample Buffer delivers a convenient and effective solution for denaturing and preparing protein samples for SDS-PAGE. With its proven formulation and ease of use, it is an essential reagent for consistent and high-quality protein electrophoresis results in research and diagnostic labs.